RNF126 Rekombinanter Antikörper

RNF126 Rekombinant Antikörper für WB, IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242215A10

Kat-Nr. : 84772-6-PBS

Synonyme

242215A10, E3 ubiquitin-protein ligase RNF126, EC:2.3.2.27, RING finger protein 126

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  WB analysis using 84772-6-RR (same clone as 84772-6-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84772-6-RR (RNF126 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84772-6-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 84772-6-RR (same clone as 84772-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84772-6-PBS in a different storage buffer formulation.

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  IHC staining of human placenta using 84772-6-RR (same clone as 84772-6-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84772-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse ovary using 84772-6-RR (same clone as 84772-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84772-6-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 84772-6-RR (same clone as 84772-6-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84772-6-PBS in a different storage buffer formulation.

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  IHC staining of rat pancreas using 84772-6-RR (same clone as 84772-6-PBS)

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84772-6-PBS in a different storage buffer formulation.

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  FC experiment of Jurkat using 84772-6-RR (same clone as 84772-6-PBS)

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug RNF126 Recombinant antibody (84772-6-RR, Clone:242215A10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84772-6-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84772-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84772-6-RR against Human RNF126 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

84772-6-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA RNF126 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RNF126 fusion protein Ag13816
• Vollständiger Name: ring finger protein 126
• Berechnetes Molekulargewicht: 326 aa, 36 kDa
• Beobachtetes Molekulargewicht: 36 kDa
• GenBank-Zugangsnummer: BC001442
• Gene symbol: RNF126
• Gene ID (NCBI): 55658
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator).