RNF135 Polyklonaler Antikörper

RNF135 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 25061-1-AP

Synonyme

RIG-I E3 ubiquitin ligase, RIG I E3 ubiquitin ligase, REUL, L13, EC:2.3.2.27

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  WB analysis of mouse kidney using 25061-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25061-1-AP (RNF135 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 25061-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25061-1-AP (RNF135 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 25061-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25061-1-AP (RNF135 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human kidney using 25061-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25061-1-AP (RNF135 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human stomach using 25061-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 25061-1-AP (RNF135 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 25061-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25061-1-AP (RNF135 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human stomach using 25061-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 25061-1-AP (RNF135 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of PC-12 using 25061-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using RNF135 antibody (25061-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HEK-293 using 25061-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RNF135 antibody (25061-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausnierengewebe, U-87 MG-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: PC-12-Zellen, HEK-293-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:1000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below
• IHC: See 2 publications below

Produktinformation

25061-1-AP bindet in WB, IHC, IF/ICC, ELISA RNF135 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF135 fusion protein Ag18821
• Vollständiger Name: ring finger protein 135
• Berechnetes Molekulargewicht: 432 aa, 48 kDa
• Beobachtetes Molekulargewicht: 48 kDa
• GenBank-Zugangsnummer: BC005084
• Gene symbol: RNF135
• Gene ID (NCBI): 84282
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ring finger protein 135 (RNF135), located on chromosome 17q11.2, is a RING finger domain-containing E3 ubiquitin ligase that was identified as a bio-marker and therapy target of glioblastoma (PMID: 26856755). RNF135 facilitates RIG-I C-terminal ubiquitination to up-regulate RIG-I-mediated IFN signaling and suppress viral replication. This antibody can detect a band at 48 kDa as predicted.

Publikationen

Species	Application	Title
human	WB	J Biol Chem; A RIG-I-like receptor directs antiviral responses to a bunyavirus and is antagonized by virus-induced blockade of TRIM25-mediated ubiquitination.; Authors - Yuan-Qin Min
human	WB	Oncol Lett; Ubiquitination of p21 by E3 ligase RNF135 promotes the proliferation of human glioblastoma cells; Authors - Weiting Gu
human	WB,IHC	Cancer Rep (Hoboken); RNF135 Promotes Human Osteosarcoma Cell Growth and Inhibits Apoptosis by Upregulating the PI3K/AKT Pathway; Authors - Bingyao Chen
human	WB,IHC	Environ Toxicol; Epigenetic Regulation of RNF135 by LSD1 Promotes Stemness Maintenance and Brain Metastasis in Lung Adenocarcinoma; Authors - Xiaohan Qu