RNF139 Rekombinanter Antikörper

RNF139 Rekombinant Antikörper für WB, FC (Intra), ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), ELISA

Konjugation

Unkonjugiert

CloneNo.

240481G6

Kat-Nr. : 83755-2-PBS

Synonyme

240481G6, E3 ubiquitin-protein ligase RNF139, EC:2.3.2.27, HRCA1, RCA1

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  WB analysis using 83755-2-RR (same clone as 83755-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83755-2-RR (RNF139 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83755-2-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 83755-2-RR (same clone as 83755-2-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug Rnf139 Recombinant Antibody (83755-2-RR, Clone:240481G6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83755-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83755-2-PBS bindet in WB, FC (Intra), ELISA RNF139 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RNF139 fusion protein Ag21530
• Vollständiger Name: ring finger protein 139
• Berechnetes Molekulargewicht: 664 aa, 76 kDa
• Beobachtetes Molekulargewicht: 76 kDa
• GenBank-Zugangsnummer: BC064636
• Gene symbol: RNF139
• Gene ID (NCBI): 11236
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The RNF139 gene (ring-finger protein 139), also known as TRC8 (translocation in renal carcinoma from chromosome 8), was first identified from a family with 3;8 chromosomal translocation and hereditary renal cell carcinoma and thyroid cancer (PMID:22689053). RNF139 encodes an endoplasmic reticulum (ER) protein with 10 transmembrane segments that contain a sterol-sensing domain and a RING finger motif encoding an E3 ubiquitin ligase (PMID: 19706601). Northern blot and dot blot show that RNF139 is highly expressed in the testis, placenta, and adrenal gland and is expressed at lower levels in the heart, brain, liver, skeletal muscle, and pancreas (PMID: 9689122). It is reported that TRC8 suppresses tumorigenesis by targeting heme oxygenase-1 (HO-1), an antioxidant enzyme highly expressed in various cancers, for ubiquitination and degradation (PMID:22689053). TRC8 is also involved in cholesterol and fatty acid biosynthesis that are transcriptionally regulated by the sterol response element binding proteins (SREBPs) (PMID:17016439). Acetylation, phosphorylation, and autoubiquitination are common post-translational modifications of FNF139 protein.