RNF144A Polyklonaler Antikörper

RNF144A Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

hamster, human

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 26144-1-AP

Synonyme

UbcM4-interacting protein 4, UBCE7IP4, RNF144, KIAA0161, EC:2.3.2.31

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  WB analysis of MDA-MB-231 using 26144-1-AP

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 26144-1-AP (RNF144A antibody) at dilution of 1:1500 and incubated at room temperature for 1.5 hours.

• 

  IP experiment of CHO using 26144-1-AP

  IP result of anti-RNF144A (IP:26144-1-AP, 4ug; Detection:26144-1-AP 1:300) with CHO cells lysate 3100ug.

• 

  IP experiment of CHO using 26144-1-AP

  IP result of anti-RNF144A (IP:26144-1-AP, 4ug; Detection:26144-1-AP 1:300) with CHO cells lysate 3080ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-231-Zellen
• Erfolgreiche IP: CHO-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

26144-1-AP bindet in WB, IP, ELISA RNF144A und zeigt Reaktivität mit hamster, human

• Getestete Reaktivität: hamster, human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF144A fusion protein Ag23508
• Vollständiger Name: ring finger protein 144A
• Beobachtetes Molekulargewicht: 33 kDa
• GenBank-Zugangsnummer: BC050373
• Gene symbol: RNF144A
• Gene ID (NCBI): 9781
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF144A (Ring Finger protein 144A) as the first E3 ubiquitin ligase for cytosolic DNA-PKcs, is induced in a p53-dependent manner during DNA damage and targets cytosolic DNA-PKcs for ubiquitination and degradation. RNF144A is an RBR-containing protein, and is unstable and degraded through the proteasome-dependent pathway (PMID: 24979766). RNF144A contains an RBR domain in the N-terminus and a potential single-transmembrane (TM) domain in the C-terminus, and is distributed in both plasma membrane and the membranes of intracellular vesicles including endosomes, lysosomes, endoplasmic reticulum (ER), perinuclear vesicles, and others, which might be caused by the presence of the TM domain (PMID: 37955227).

Publikationen

Species	Application	Title
human	WB	EMBO Rep; RNF144A promotes antiviral responses by modulating STING ubiquitination; Authors - Bo Yang
human	WB	Autophagy; RNF144A inhibits autophagy by targeting BECN1 for degradation during L. monocytogenes infection; Authors - Bo Yang