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RNF144A Polyklonaler Antikörper

RNF144A Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

hamster, human

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26144-1-PBS

Synonyme

UbcM4-interacting protein 4, UBCE7IP4, RNF144, KIAA0161, EC:2.3.2.31

Filter:
  • Western Blot (WB) analysis of MDA-MB-231 cells using RNF144A Polyclonal antibody (26144-1-AP)

    WB analysis of MDA-MB-231 using 26144-1-AP (same clone as 26144-1-PBS)

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 26144-1-AP (RNF144A antibody) at dilution of 1:1500 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 26144-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of CHO cells using RNF144A Polyclonal antibody (26144-1-AP)

    IP experiment of CHO using 26144-1-AP (same clone as 26144-1-PBS)

    IP result of anti-RNF144A (IP:26144-1-AP, 4ug; Detection:26144-1-AP 1:300) with CHO cells lysate 3100ug. This data was developed using the same antibody clone with 26144-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of CHO cells using RNF144A Polyclonal antibody (26144-1-AP)

    IP experiment of CHO using 26144-1-AP (same clone as 26144-1-PBS)

    IP result of anti-RNF144A (IP:26144-1-AP, 4ug; Detection:26144-1-AP 1:300) with CHO cells lysate 3080ug. This data was developed using the same antibody clone with 26144-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

26144-1-PBS bindet in WB, IP, Indirect ELISA RNF144A und zeigt Reaktivität mit hamster, human

Getestete Reaktivität hamster, human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RNF144A fusion protein Ag23508
Vollständiger Name ring finger protein 144A
Beobachtetes Molekulargewicht 33 kDa
GenBank-ZugangsnummerBC050373
Gene symbol RNF144A
Gene ID (NCBI) 9781
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF144A (Ring Finger protein 144A) as the first E3 ubiquitin ligase for cytosolic DNA-PKcs, is induced in a p53-dependent manner during DNA damage and targets cytosolic DNA-PKcs for ubiquitination and degradation. RNF144A is an RBR-containing protein, and is unstable and degraded through the proteasome-dependent pathway (PMID: 24979766). RNF144A contains an RBR domain in the N-terminus and a potential single-transmembrane (TM) domain in the C-terminus, and is distributed in both plasma membrane and the membranes of intracellular vesicles including endosomes, lysosomes, endoplasmic reticulum (ER), perinuclear vesicles, and others, which might be caused by the presence of the TM domain (PMID: 37955227).