RNF151 Polyklonaler Antikörper

RNF151 Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 24708-1-AP

Synonyme

ring finger protein 151, RNF151

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Galerie der Validierungsdaten

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  WB analysis of rat testis using 24708-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 24708-1-AP (RNF151 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 24708-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 24708-1-AP (RNF151 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 24708-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24708-1-AP (RNF151 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse testis using 24708-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24708-1-AP (RNF151 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 24708-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 24708-1-AP (RNF151 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattenhodengewebe, humanes Hodengewebe, Maushodengewebe
• Erfolgreiche Detektion in IHC: Maushodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

24708-1-AP bindet in WB, IHC, ELISA RNF151 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF151 fusion protein Ag16606
• Vollständiger Name: ring finger protein 151
• Berechnetes Molekulargewicht: 245 aa, 27 kDa
• Beobachtetes Molekulargewicht: 26 kDa
• GenBank-Zugangsnummer: BC113014
• Gene symbol: RNF151
• Gene ID (NCBI): 146310
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF151 also termed as ring finger protein 151 is a 245 amino acid protein, which contains one Ring-type zinc finger and one TRAF-type zinc finger. RNF151, with, a putative nuclear localization signal (NLS)was exclusively expressed in the mouse testis and developmentally regulated during spermatogenesis. While RNF151 mRNA was present in round spermatids, its protein was expressed in elongating spermatids of the stage VIII-IX seminiferous tubules.