RNF160 Polyklonaler Antikörper

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• KD/KO Validated

RNF160 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Kat-Nr. : 28452-1-AP

Synonyme

C21orf10, C21orf98, E3 ubiquitin-protein ligase listerin, EC:2.3.2.27, HSPC087

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  WB analysis using 28452-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28452-1-AP (RNF160 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 28452-1-AP

  WB result of RNF160 antibody (28452-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF160 transfected HepG2 cells.

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  WB analysis using 28452-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28452-1-AP (RNF160 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 28452-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28452-1-AP (RNF160 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 28452-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using RNF160 antibody (28452-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen, HepG2-Zellen, Maus-Pankreasgewebe, Ratten-Pankreasgewebe
• Erfolgreiche Detektion in IHC: Maus-Kolongewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below

Produktinformation

28452-1-AP bindet in WB, IHC, IF/ICC, ELISA RNF160 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF160 fusion protein Ag29467
• Vollständiger Name: ring finger protein 160
• Beobachtetes Molekulargewicht: 180-200 kDa
• GenBank-Zugangsnummer: BC140790
• Gene symbol: RNF160
• Gene ID (NCBI): 26046
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The E3 ubiquitin ligase, Ltn1 (also named RNF160), is a key regulator of RQC that targets substrates for ubiquitination (PMID: 35981909).

Publikationen

Species	Application	Title
human		Nat Commun; Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis; Authors - Ji Geng
human	WB	Cell Rep; NEMF-mediated Listerin-independent mitochondrial translational surveillance by E3 ligase Pirh2 and mitochondrial protease ClpXP; Authors - Liang Lv
human	WB	Acta Neuropathol Commun; Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease; Authors - Suman Rimal
human		Cell Rep; The E3 ubiquitin ligase FBXL6 controls the quality of newly synthesized mitochondrial ribosomal proteins; Authors - Julie Lavie
human	WB	bioRxiv; Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats; Authors - Yi-Ju Tseng
human	WB	bioRxiv; Nascent Chain Ubiquitination is Uncoupled from Degradation to Enable Protein Maturation; Authors - Xia Li