RNF160 Rekombinanter Antikörper

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• KD/KO Validated

RNF160 Rekombinant Antikörper für WB, IHC, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230075H2

Kat-Nr. : 82926-1-PBS

Synonyme

HSPC087, EC:2.3.2.27, E3 ubiquitin-protein ligase listerin, C21orf98, C21orf10

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  WB analysis using 82926-1-RR (same clone as 82926-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82926-1-RR (RNF160 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 82926-1-RR (same clone as 82926-1-PBS)

  WB result of RNF160 antibody (82926-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF160 transfected HepG2 cells. This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 82926-1-RR (same clone as 82926-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82926-1-RR (RNF160 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 82926-1-RR (same clone as 82926-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug RNF160 Recombinant antibody (82926-1-RR, Clone:230075H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82926-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82926-1-RR against Human ADAMDEC1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

82926-1-PBS bindet in WB, IHC, FC (Intra), Indirect ELISA RNF160 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RNF160 fusion protein Ag29467
• Vollständiger Name: ring finger protein 160
• Beobachtetes Molekulargewicht: 180-200 kDa
• GenBank-Zugangsnummer: BC140790
• Gene symbol: RNF160
• Gene ID (NCBI): 26046
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The E3 ubiquitin ligase, Ltn1 (also named RNF160), is a key regulator of RQC that targets substrates for ubiquitination (PMID: 35981909).