RNF167 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RNF167 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 24618-1-AP

Synonyme

E3 ubiquitin-protein ligase RNF167, EC:2.3.2.27, LP2254, RING finger protein 167, RING105

---

• 

  WB analysis of SH-SY5Y using 24618-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 24618-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 24618-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 24618-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24618-1-AP (RNF167 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HaCaT using 24618-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using RNF167 antibody (24618-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-231-Zellen, HeLa-Zellen, MCF-7-Zellen, Maushirngewebe, Maushodengewebe, SH-SY5Y-Zellen
• Erfolgreiche Detektion in IF/ICC: HaCaT-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IP: See 1 publications below

Produktinformation

24618-1-AP bindet in WB, IF/ICC, IP, ELISA RNF167 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF167 fusion protein Ag18383
• Vollständiger Name: ring finger protein 167
• Berechnetes Molekulargewicht: 350 aa, 38 kDa
• Beobachtetes Molekulargewicht: 46-50 kDa
• GenBank-Zugangsnummer: BC010139
• Gene symbol: RNF167
• Gene ID (NCBI): 26001
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF167 also named as, ring finger protein 167 or LP2254, is a 350 amino acid protein, which contains one protease associated domain and one Ring-type zinc finger. RNF167 as single-pass membrane protein localizes in the endomembrane system and is strongly expressed in the kidney and liver. RNF167 may act as an E3 ubiquitin-protein ligase,or as a part of the E3 complex, which accepts ubiquitin from specific E3 ubiquitin from ubiquitin conjugation enzymes. RNF167 also may play a role in growth regulation in G1/S transition. The molecular weight of RNF167 is 38 kDa, but we detected a 47 kDa protein by western blot. Our result is similar to the figure of one research paper (PMID:23129617).

Publikationen

Species	Application	Title
human	WB	Nat Commun; RNF167 activates mTORC1 and promotes tumorigenesis by targeting CASTOR1 for ubiquitination and degradation.; Authors - Tingting Li ; KD Validated
human	WB	FASEB J; RNF167-mediated ubiquitination of Tollip inhibits TNF-α-triggered NF-κB and MAPK activation; Authors - Zhenzhen Yan
mouse,human	WB,IP	Nat Commun; RNF167 mediates atypical ubiquitylation and degradation of RLRs via two distinct proteolytic pathways; Authors - Miao He ; KD Validated