RNF168 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RNF168 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Rezensionen (2)

Kat-Nr. : 21393-1-AP

Synonyme

RING-type E3 ubiquitin transferase RNF168, RING finger protein 168, hRNF168, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF168

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  WB analysis of HepG2 using 21393-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 21393-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Transfected HEK-293 using 21393-1-AP

  Transfected HEK-293 cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 Antibody) at dilution of 1:500 incubated at 4 degree celsius over night.

• 

  WB analysis of HeLa using 21393-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21393-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21393-1-AP (RNF168 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 21393-1-AP

  IP result of anti-RNF168 (IP:21393-1-AP, 4ug; Detection:21393-1-AP 1:500) with HepG2 cells lysate 2400ug.

• 

  IF Staining of HepG2 using 21393-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNF168 antibody (21393-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HeLa-Zellen, humanes Hirngewebe, Jurkat-Zellen, Transfizierte HEK-293-Zellen
• Erfolgreiche IP: HepG2-Zellen
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 1 publications below
• IF: See 4 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

21393-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA RNF168 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF168 fusion protein Ag16055
• Vollständiger Name: ring finger protein 168
• Berechnetes Molekulargewicht: 571 aa, 65 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC033791
• Gene symbol: RNF168
• Gene ID (NCBI): 165918
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
		Mol Cell; A di-acetyl-decorated chromatin signature couples liquid condensation to suppress DNA end synapsis; Authors - Kaiwen Bao
human	WB,IHC,IF	Cell Death Differ; ZNF451 collaborates with RNF8 to regulate RNF168 localization and amplify ubiquitination signaling to promote DNA damage repair and regulate radiosensitivity; Authors - Feng Xu
human	IP	Nat Commun; Non-canonical function of DGCR8 in DNA double-strand break repair signaling and tumor radioresistance.; Authors - Qinglei Hang
human		Nucleic Acids Res; GLP-catalyzed H4K16me1 promotes 53BP1 recruitment to permit DNA damage repair and cell survival.; Authors - Xiaopeng Lu
human	WB	Theranostics; A feedforward circuit shaped by ECT2 and USP7 contributes to breast carcinogenesis.; Authors - Qi Zhang
human	WB,IF	J Cell Biol; DUX4-induced HSATII transcription causes KDM2A/B-PRC1 nuclear foci and impairs DNA damage response; Authors - Tessa Arends

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Shunbin (Verified Customer) (05-14-2024)

Great. A single specific band, no background

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: B cell lines

RNF168 Antibody Western Blot validation (1:1000 dilution) in B cell lines (Cat no:21393-1-AP)

FH

Shunbin (Verified Customer) (05-14-2024)

Great. A single specific band without background

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: B cell lines

RNF168 Antibody Western Blot validation (1:1000 dilution) in B cell lines (Cat no:21393-1-AP)