RNF180 Polyklonaler Antikörper

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• KD/KO Validated

RNF180 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30941-1-PBS

Synonyme

E3 ubiquitin-protein ligase RNF180, EC:2.3.2.27, RINES, RING finger protein 180, RNF 180

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  WB analysis using 30941-1-AP (same clone as 30941-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 30941-1-AP (RNF180 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30941-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 30941-1-AP (same clone as 30941-1-PBS)

  WB result of RNF180 antibody (30941-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF180 transfected A549 cells. This data was developed using the same antibody clone with 30941-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 30941-1-AP (same clone as 30941-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 30941-1-AP (RNF180 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 30941-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 30941-1-AP (same clone as 30941-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RNF180 antibody (30941-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 30941-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

30941-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RNF180 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF180 fusion protein Ag34138
• Vollständiger Name: ring finger protein 180
• Berechnetes Molekulargewicht: 592 aa, 68 kDa
• Beobachtetes Molekulargewicht: 70 kDa,48kDa
• GenBank-Zugangsnummer: BC101277
• Gene symbol: RNF180
• Gene ID (NCBI): 285671
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.