RNF181 Polyklonaler Antikörper

RNF181 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 20408-1-AP

Synonyme

RN181, RING finger protein 181, HSPC238, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF181

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  WB analysis of Jurkat using 20408-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 20408-1-AP (RNF181 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 20408-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20408-1-AP (RNF181 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 20408-1-AP

  IP result of anti-RNF181 (IP:20408-1-AP, 3ug; Detection:20408-1-AP 1:500) with Jurkat cells lysate 1200ug.

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  IHC staining of human kidney using 20408-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 20408-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 20408-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human heart using 20408-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20408-1-AP (RNF181 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 20408-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF181 antibody (20408-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, L02-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Herzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

20408-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA RNF181 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF181 fusion protein Ag14233
• Vollständiger Name: ring finger protein 181
• Berechnetes Molekulargewicht: 153 aa, 18 kDa
• Beobachtetes Molekulargewicht: 18 kDa
• GenBank-Zugangsnummer: BC002803
• Gene symbol: RNF181
• Gene ID (NCBI): 51255
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF181, also named as HSPC238, is a 153 amino acid protein, which belongs to the RNF181 family. RNF181 is widely expressed, with highest levels in liver and heart and lowest levels in brain and skeletal muscle. RNF181 as a E3 ubiquitin-protein ligase, accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Western blot analysis detected RNF181 protein in human platelets as a 36-kD dimer and an 18-kD monomer.

Publikationen

Species	Application	Title
	WB	Oncol Rep; Screening and verification of proteins that interact with HSPC238.; Authors - Jia-Yu Tan