RNF181 Rekombinanter Antikörper

RNF181 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

243121B5

Kat-Nr. : 85735-2-RR

Synonyme

E3 ubiquitin-protein ligase RNF181, EC:2.3.2.27, HSPC238, RING finger protein 181, RN181

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  WB analysis using 85735-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85735-2-RR (RNF181 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of Jurkat using 85735-2-RR

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using RNF181 antibody (85735-2-RR, Clone: 243121B5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 85735-2-RR

  Biolayer interferometry (BLl) kinetic assays of 85735-2-RR against Human RNF181 were performed. The affinity constant is 0.138 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, A549-Zellen, HEK-293-Zellen, Jurkat-Zellen, U2OS-Zellen
• Erfolgreiche Detektion in IF/ICC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85735-2-RR bindet in WB, IF/ICC, ELISA RNF181 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RNF181 fusion protein Ag14233
• Vollständiger Name: ring finger protein 181
• Berechnetes Molekulargewicht: 153 aa, 18 kDa
• Beobachtetes Molekulargewicht: 20 kDa
• GenBank-Zugangsnummer: BC002803
• Gene symbol: RNF181
• Gene ID (NCBI): 51255
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF181, also named as HSPC238, is a 153 amino acid protein, which belongs to the RNF181 family. RNF181 is widely expressed, with highest levels in liver and heart and lowest levels in brain and skeletal muscle. RNF181 as a E3 ubiquitin-protein ligase, accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates.