RNF183 Polyklonaler Antikörper

RNF183 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32715-1-AP

Synonyme

E3 ubiquitin-protein ligase RNF183, EC:2.3.2.27

---

• 

  WB analysis using 32715-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32715-1-AP (RNF183 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: 5637 cells, HCT 116-Zellen, HT-29-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32715-1-AP bindet in WB, ELISA RNF183 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF183 fusion protein Ag36228
• Vollständiger Name: ring finger protein 183
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC013036
• Gene symbol: RNF183
• Gene ID (NCBI): 138065
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF183, or Ring Finger Protein 183, is a member of the RING finger protein family that functions as a ubiquitin ligase. RNF183 exhibits tissue-specific expression patterns, with higher expression levels in the gall bladder, kidney, and other tissues. RNF183 expression levels are increased in patients with IBD, including Crohn's disease and ulcerative colitis (PMID: 32486221). It promotes intestinal inflammation by increasing the ubiquitination and degradation of IkappaBalpha, thereby inducing NF-kappaB activation (PMID: 31889078). RNF183 has also been implicated in other conditions such as chronic kidney disease and Alzheimer's disease.