RNF2 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RNF2 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16031-1-PBS

Synonyme

HIP2 interacting protein 3, EC:2.3.2.27, E3 ubiquitin-protein ligase RING2, DING, BAP1

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  WB analysis of K-562 using 16031-1-AP (same clone as 16031-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16031-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 16031-1-AP (same clone as 16031-1-PBS)

  WB result of RNF2 antibody (16031-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF2 transfected HEK-293 cells. This data was developed using the same antibody clone with 16031-1-PBS in a different storage buffer formulation.

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  WB analysis of U-937 using 16031-1-AP (same clone as 16031-1-PBS)

  U-937 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16031-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 16031-1-AP (same clone as 16031-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16031-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 16031-1-AP (same clone as 16031-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16031-1-AP (RNF2 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16031-1-PBS in a different storage buffer formulation.

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  IP experiment of K-562 using 16031-1-AP (same clone as 16031-1-PBS)

  IP result of anti-RNF2 (IP:16031-1-AP, 4ug; Detection:16031-1-AP 1:800) with K-562 cells lysate 1200ug. This data was developed using the same antibody clone with 16031-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 16031-1-AP (same clone as 16031-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 16031-1-AP (RNF2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16031-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16031-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA RNF2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF2 fusion protein Ag8900
• Vollständiger Name: ring finger protein 2
• Berechnetes Molekulargewicht: 336 aa, 38 kDa
• Beobachtetes Molekulargewicht: 38 kDa
• GenBank-Zugangsnummer: BC012583
• Gene symbol: RNF2
• Gene ID (NCBI): 6045
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF2 (also known as ding, Ring1B or Ring2) is a member of the Ring finger protein family, which functions as E3 ubiquitin ligase for monoubiquitination of histone H2A at lysine 119 (H2AK119ub). Many studies have demonstrated that overexpressed RNF2 was involved in the pathological progression of multiple cancers and has an impact on their clinical features. For instance, the upregulated expression level of RNF2 is positively correlated with the occurrence and progression of hepatocellular carcinoma, melanoma, prostate cancer, breast cancer, pancreatic cancer, gastric cancer, and bladder urothelial carcinoma, as well as with the radioresistance of lung cancer and chemoresistance of ovarian cancer (PMID: 33400276). The molecular weight of RNF2 is 38 kDa.