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RNF20 Rekombinanter Antikörper

RNF20 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243078G12

Kat-Nr. : 85630-6-PBS

Synonyme

BRE1, BRE1 A, BRE1A, BRE1-A, E3 ubiquitin-protein ligase BRE1A

Filter:
  • Western Blot (WB) analysis of various lysates using RNF20 Recombinant antibody (85630-6-RR)

    WB analysis using 85630-6-RR (same clone as 85630-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85630-6-RR (RNF20 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85630-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-87 MG cells using RNF20 Recombinant antibody (85630-6-RR)

    IF Staining of U-87 MG using 85630-6-RR (same clone as 85630-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U-87 MG cells using RNF20 antibody (85630-6-RR, Clone: 243078G12 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85630-6-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

85630-6-PBS bindet in WB, IF/ICC, Indirect ELISA RNF20 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen RNF20 fusion protein Ag16066
Vollständiger Name ring finger protein 20
Berechnetes Molekulargewicht 975 aa, 114 kDa
Beobachtetes Molekulargewicht114 kDa
GenBank-ZugangsnummerBC110585
Gene symbol RNF20
Gene ID (NCBI) 56254
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF20, also named as E3 ubiquitin-protein ligase BRE1A, is a 975 amino acid protein, which contains 1 RING-type zinc finger and belongs to the BRE1 family. RNF20 is a component of the RNF20/40 complex and localizes in the nucleus. RNF20 is a component of the RNF20/40 E3 ubiquitin-protein ligase complex that mediates monoubiquitination of 'Lys-120' of histone H2B (H2BK120ub1). H2BK120ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation (H3K4me and H3K79me, respectively). The RNF20/40 complex forms a H2B ubiquitin ligase complex in cooperation with the E2 enzyme UBE2A or UBE2B. RNF20 is required for transcriptional activation of Hox genes and recruited to the MDM2 promoter, probably by being recruited by p53/TP53, and thereby acts as a transcriptional coactivator. RNF20/40 complex monoubiquitinates and stabilizes Eg5. Loss of RNF20/40 results in spindle assembly defects, cell cycle arrest and apoptosis. Spindle assembly role of the RNF20/40 complex, and implicates the RNF20/40-Eg5 axis in breast carcinogenesis, supporting the pursuit of these proteins as potential targets for breast cancer therapeutic interventions.