RNF220 Polyklonaler Antikörper

RNF220 Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 32482-1-AP

Synonyme

RING-type E3 ubiquitin transferase RNF220, RING finger protein 220, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF220, C1orf164

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  WB analysis using 32482-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 32482-1-AP (RNF220 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: RT-4 cells, Maushirngewebe, Rattenhirngewebe, U-251-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

32482-1-AP bindet in WB, ELISA RNF220 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF220 fusion protein Ag36190
• Vollständiger Name: ring finger protein 220
• Beobachtetes Molekulargewicht: 70 kDa
• GenBank-Zugangsnummer: BC034221
• Gene symbol: RNF220
• Gene ID (NCBI): 55182
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF220 is an E3 ubiquitin ligase that plays a crucial role in the ubiquitination process, which targets proteins for degradation by the proteasome. Mutations in RNF220 have been linked to laminopathies, which are characterized by leukodystrophy, ataxia, and deafness. Additionally, RNF220 is involved in the progression of certain cancers, such as acute myeloid leukemia (AML) and colorectal cancer. Studies have shown that RNF220 promotes the proliferation of leukemia cells and reduces the degradation of Cyclin D1 protein through USP22. It also enhances canonical Wnt signaling through USP7-mediated deubiquitination of beta-catenin. (PMID: 32896826)