RNF31 / HOIP Rekombinanter Antikörper

RNF31 / HOIP Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230446H1

Kat-Nr. : 83137-3-PBS

Synonyme

HOIP, RNF31, 230446H1, E3 ubiquitin-protein ligase RNF31, EC:2.3.2.31

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  IF Staining of HepG2 using 83137-3-RR (same clone as 83137-3-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF31 antibody (83137-3-RR, Clone: 230446H1 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83137-3-PBS in a different storage buffer formulation.

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  FC experiment of PC-3 using 83137-3-RR (same clone as 83137-3-PBS)

  1x10^6 PC-3 cells were intracellularly stained with 0.2 ug Rnf31 Recombinant Antibody (83137-3-RR, Clone:230446H1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83137-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00285-1

  Cytometric bead array standard curve of MP00285-1, RNF31 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83137-3-PBS. Detection antibody: 83137-1-PBS. Standard: Ag34505. Range: 1.25-20 ng/mL.

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  Cytometric bead array standard curve of MP00285-2

  Cytometric bead array standard curve of MP00285-2, RNF31 Recombinant Matched Antibody Pair - PBS only. Capture antibody: 83137-3-PBS. Detection antibody: 83137-2-PBS. Standard: Ag34505. Range: 1.25-20 ng/mL.

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  Affinity and Kinetic Characterization of 83137-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83137-3-RR against Human RNF31 / HOIP were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83137-3-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA RNF31 / HOIP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RNF31 / HOIP fusion protein Ag34505
• Vollständiger Name: ring finger protein 31
• Berechnetes Molekulargewicht: 1072 aa, 120 kDa
• GenBank-Zugangsnummer: BC017376
• Gene symbol: RNF31
• Gene ID (NCBI): 55072
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.