RNF31 / HOIP Rekombinanter Antikörper

RNF31 / HOIP Rekombinant Antikörper für WB, FC (Intra), Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

230446E9

Kat-Nr. : 83137-4-PBS

Synonyme

HOIP, RNF31, 230446E9, E3 ubiquitin-protein ligase RNF31, EC:2.3.2.31

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  WB analysis using 83137-4-RR (same clone as 83137-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83137-4-RR (RNF31 / HOIP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83137-4-PBS in a different storage buffer formulation.

• 

  FC experiment of PC-3 using 83137-4-RR (same clone as 83137-4-PBS)

  1x10^6 PC-3 cells were intracellularly stained with 0.2 ug RNF31 / HOIP Recombinant antibody (83137-4-RR, Clone:230446E9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.2 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83137-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

83137-4-PBS bindet in WB, FC (Intra), Indirect ELISA RNF31 / HOIP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RNF31 / HOIP fusion protein Ag34505
• Vollständiger Name: ring finger protein 31
• Berechnetes Molekulargewicht: 1072 aa, 120 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC017376
• Gene symbol: RNF31
• Gene ID (NCBI): 55072
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.