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RNF31 / HOIP Polyklonaler Antikörper

RNF31 / HOIP Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 30797-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using RNF31 / HOIP Polyclonal antibody (30797-1-AP)

    WB analysis using 30797-1-AP (same clone as 30797-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30797-1-AP (RNF31 / HOIP antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30797-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using RNF31 / HOIP Polyclonal antibody (30797-1-AP)

    WB analysis of HEK-293 using 30797-1-AP (same clone as 30797-1-PBS)

    WB result of RNF31 / HOIP antibody (30797-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF31 / HOIP transfected HEK-293 cells. This data was developed using the same antibody clone with 30797-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RNF31 / HOIP Polyclonal antibody (30797-1-AP)

    IF Staining of HepG2 using 30797-1-AP (same clone as 30797-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using RNF31 / HOIP antibody (30797-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 30797-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

30797-1-PBS bindet in WB, IF/ICC, Indirect ELISA RNF31 / HOIP und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RNF31 / HOIP fusion protein Ag34505
Vollständiger Name ring finger protein 31
Berechnetes Molekulargewicht 1072 aa, 120 kDa
Beobachtetes Molekulargewicht 100 kDa
GenBank-ZugangsnummerBC017376
Gene symbol RNF31
Gene ID (NCBI) 55072
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Ring finger protein 31 (RNF31), also named HOIL-1-interacting protein (HOIP), ZIBRA, is an atypical E3 ubiquitin ligase, belongs to ring finger protein (RNF) family. It was first cloned from breast cancer cell line MCF7 and was identified as a classical component in linear ubiquitin assembly complex (LUBAC) to facilitate NFκB signaling transduction. It also plays important regulatory functions in controlling important oncogenic pathways in breast cancer, such as ERα and p53. RNF31 is highly expressed in muscle, heart, and testis. In cells, RNF31 mainly localizes to the cytoplasm. (PMID:27460922)