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RNF38 Polyklonaler Antikörper

RNF38 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 25132-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of HepG2 cells using RNF38 Polyclonal antibody (25132-1-AP)

    WB analysis of HepG2 using 25132-1-AP (same clone as 25132-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 25132-1-AP (RNF38 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25132-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using RNF38 Polyclonal antibody (25132-1-AP)

    IHC staining of human endometrial cancer using 25132-1-AP (same clone as 25132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25132-1-AP (RNF38 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25132-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using RNF38 Polyclonal antibody (25132-1-AP)

    IHC staining of human endometrial cancer using 25132-1-AP (same clone as 25132-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 25132-1-AP (RNF38 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25132-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using RNF38 Polyclonal antibody (25132-1-AP)

    IF Staining of HepG2 using 25132-1-AP (same clone as 25132-1-PBS)

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 25132-1-AP (RNF38 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 25132-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

25132-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RNF38 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RNF38 fusion protein Ag16054
Vollständiger Name ring finger protein 38
Berechnetes Molekulargewicht 515 aa, 58 kDa
Beobachtetes Molekulargewicht 43-58 kDa
GenBank-ZugangsnummerBC033786
Gene symbol RNF38
Gene ID (NCBI) 152006
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RING finger protein 38 is a protein that in humans is encoded by the RNF38 gene.This gene encodes a protein with a coiled-coil motif and a RING-H2 motif (C3H2C2) at its carboxy-terminus. The RING motif is a zinc-binding domain found in a large set of proteins playing roles in diverse cellular processes including oncogenesis, development, signal transduction, and apoptosis. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. RNF38 is widely expressed with highest levels in testis. RNF38 exists various isoforms and molecular weight of isoforms are 36, 48, 52 and 58 kDa