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RNF4 Polyklonaler Antikörper

RNF4 Polyklonal Antikörper für WB, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17810-1-PBS

Synonyme

RES4-26, RES4 26, Protein SNURF, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF4

Filter:
  • Western Blot (WB) analysis of PC-3 cells using RNF4 Polyclonal antibody (17810-1-AP)

    WB analysis of PC-3 using 17810-1-AP (same clone as 17810-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 17810-1-AP (RNF4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17810-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of PC-3 cells using RNF4 Polyclonal antibody (17810-1-AP)

    IP experiment of PC-3 using 17810-1-AP (same clone as 17810-1-PBS)

    IP result of anti-RNF4 (IP:17810-1-AP, 4ug; Detection:17810-1-AP 1:1500) with PC-3 cells lysate 1800 ug. This data was developed using the same antibody clone with 17810-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

17810-1-PBS bindet in WB, IP, Indirect ELISA RNF4 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RNF4 fusion protein Ag12115
Vollständiger Name ring finger protein 4
Berechnetes Molekulargewicht 190 aa, 21 kDa
Beobachtetes Molekulargewicht 30 kDa
GenBank-ZugangsnummerBC031935
Gene symbol RNF4
Gene ID (NCBI) 6047
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF4 is a E3 ubiquitin-protein ligase that binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. It regulates the degradation of several proteins including PML and PEA3[PMID:18408734]. It also participates in chromosome alignment and spindle assembly, and regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. In addition, RNF4 can regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 [PMID:20026589]. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation [PMID:11319220]. RNF4 exist as two isoforms. Isoform one is a 30 kDa protein and isoform two is a about 15 kDa protein. RNF4 binds polysumoylated chains and the molecular weight of RNF4 migrate in the experiment to 35 kDa, 47 kDa, 69 kDa as ubiquitinated protein.