RNF4 Rekombinanter Antikörper

RNF4 Rekombinant Antikörper für IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242197A10

Kat-Nr. : 84770-1-PBS

Synonyme

242197A10, E3 ubiquitin ligase RNF4, E3 ubiquitin-protein ligase RNF4, EC:2.3.2.27, Protein SNURF

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  IF Staining of HepG2 using 84770-1-RR (same clone as 84770-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNF4 antibody (84770-1-RR, Clone: 242197A10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84770-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 84770-1-RR (same clone as 84770-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RNF4 Recombinant antibody (84770-1-RR, Clone:242197A10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84770-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01553-1

  Cytometric bead array standard curve of MP01553-1, RNF4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84770-1-PBS. Detection antibody: 84770-2-PBS. Standard: Ag12115. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 84770-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84770-1-RR against Human RNF4 were performed. The affinity constant is 17.3 nM.

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Geprüfte Anwendungen

Produktinformation

84770-1-PBS bindet in IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA RNF4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RNF4 fusion protein Ag12115
• Vollständiger Name: ring finger protein 4
• Berechnetes Molekulargewicht: 190 aa, 21 kDa
• GenBank-Zugangsnummer: BC031935
• Gene symbol: RNF4
• Gene ID (NCBI): 6047
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF4 is a E3 ubiquitin-protein ligase that binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. It regulates the degradation of several proteins including PML and PEA3[PMID:18408734]. It also participates in chromosome alignment and spindle assembly, and regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. In addition, RNF4 can regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 [PMID:20026589]. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation [PMID:11319220].