RNF40 Polyklonaler Antikörper

RNF40 Polyklonal Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

Rezensionen (2)

Kat-Nr. : 15621-1-AP

Synonyme

EC:2.3.2.27, E3 ubiquitin-protein ligase BRE1B, BRE1-B, BRE1B, BRE1 B

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  WB analysis of HEK-293 using 15621-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15621-1-AP (RNF40 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15621-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15621-1-AP (RNF40 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 15621-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 15621-1-AP (RNF40 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, Mauslebergewebe
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

15621-1-AP bindet in WB, IF/ICC, ELISA RNF40 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF40 fusion protein Ag8047
• Vollständiger Name: ring finger protein 40
• Berechnetes Molekulargewicht: 114 kDa
• Beobachtetes Molekulargewicht: 110-140 kDa
• GenBank-Zugangsnummer: BC006133
• Gene symbol: RNF40
• Gene ID (NCBI): 9810
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The DNA damage response (DDR) is emerging as a vast signaling network that temporarily modulates numerous aspects of cellular metabolism in the face of DNA lesions, especially critical ones such as the double-strand break (DSB). The DDR involves extensive dynamics of protein post-translational modifications, most notably phosphorylation and ubiquitylation. A recently identified novel component of the DDR pathway - histone H2B monoubiquitylation - exemplifies this principle. Monoubiquitination of histone H2B is a dynamic post-translational histone modification associated with transcriptional elongation and the DNA damage response [PMID:22021426]. In mammalian cells, H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40 [PMID:22031019,21827756].

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nikolett (Verified Customer) (09-25-2025)

Antibody works well for Western Blotting after overnight incubation in 3% BSA/PBS and also in milk.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Human Lung Cancer Cell Lines

FH

Iram (Verified Customer) (03-11-2022)

This antibody works well for western

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000 for western
• Cell Tissue Type: Colon cancer cells