RNF40 Polyklonaler Antikörper
RNF40 Polyklonal Antikörper für WB, IF/ICC, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human, Maus
Anwendung
WB, IF/ICC, ELISA
Konjugation
Unkonjugiert
Kat-Nr. : 15621-1-AP
Synonyme
Geprüfte Anwendungen
Erfolgreiche Detektion in WB | HEK-293-Zellen, Mauslebergewebe |
Erfolgreiche Detektion in IF/ICC | HeLa-Zellen |
Empfohlene Verdünnung
Anwendung | Verdünnung |
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Western Blot (WB) | WB : 1:1000-1:4000 |
Immunfluoreszenz (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, check data in validation data gallery |
Produktinformation
15621-1-AP bindet in WB, IF/ICC, ELISA RNF40 und zeigt Reaktivität mit human, Maus
Getestete Reaktivität | human, Maus |
Wirt / Isotyp | Kaninchen / IgG |
Klonalität | Polyklonal |
Typ | Antikörper |
Immunogen | RNF40 fusion protein Ag8047 |
Vollständiger Name | ring finger protein 40 |
Berechnetes Molekulargewicht | 114 kDa |
Beobachtetes Molekulargewicht | 110-140 kDa |
GenBank-Zugangsnummer | BC006133 |
Gene symbol | RNF40 |
Gene ID (NCBI) | 9810 |
Konjugation | Unkonjugiert |
Form | Liquid |
Reinigungsmethode | Antigen-Affinitätsreinigung |
Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
The DNA damage response (DDR) is emerging as a vast signaling network that temporarily modulates numerous aspects of cellular metabolism in the face of DNA lesions, especially critical ones such as the double-strand break (DSB). The DDR involves extensive dynamics of protein post-translational modifications, most notably phosphorylation and ubiquitylation. A recently identified novel component of the DDR pathway - histone H2B monoubiquitylation - exemplifies this principle. Monoubiquitination of histone H2B is a dynamic post-translational histone modification associated with transcriptional elongation and the DNA damage response [PMID:22021426]. In mammalian cells, H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40 [PMID:22031019,21827756].
Protokolle
PRODUKTSPEZIFISCHE PROTOKOLLE | |
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WB protocol for RNF40 antibody 15621-1-AP | Protokoll herunterladen |
IF protocol for RNF40 antibody 15621-1-AP | Protokoll herunterladen |
STANDARD-PROTOKOLLE | |
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Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Nikolett (Verified Customer) (09-25-2025) | Antibody works well for Western Blotting after overnight incubation in 3% BSA/PBS and also in milk.
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FH Iram (Verified Customer) (03-11-2022) | This antibody works well for western
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