RNF40 Rekombinanter Antikörper

RNF40 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241214A1

Kat-Nr. : 84058-5-RR

Synonyme

E3 ubiquitin-protein ligase BRE1B, BRE1-B, BRE1B, BRE1 B, 95 kDa retinoblastoma-associated protein

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  WB analysis using 84058-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84058-5-RR (RNF40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 84058-5-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNF40 antibody (84058-5-RR, Clone: 241214A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 84058-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84058-5-RR against Human RNF40 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, A375-Zellen, HEK-293-Zellen, HepG2-Zellen
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84058-5-RR bindet in WB, IF/ICC, ELISA RNF40 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RNF40 fusion protein Ag8047
• Vollständiger Name: ring finger protein 40
• Berechnetes Molekulargewicht: 114 kDa
• Beobachtetes Molekulargewicht: 135 kDa
• GenBank-Zugangsnummer: BC006133
• Gene symbol: RNF40
• Gene ID (NCBI): 9810
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The DNA damage response (DDR) is emerging as a vast signaling network that temporarily modulates numerous aspects of cellular metabolism in the face of DNA lesions, especially critical ones such as the double-strand break (DSB). The DDR involves extensive dynamics of protein post-translational modifications, most notably phosphorylation and ubiquitylation. A recently identified novel component of the DDR pathway - histone H2B monoubiquitylation - exemplifies this principle. Monoubiquitination of histone H2B is a dynamic post-translational histone modification associated with transcriptional elongation and the DNA damage response [PMID:22021426]. In mammalian cells, H2B monoubiquitylation is driven primarily by an E3 ubiquitin ligase composed of the two RING finger proteins RNF20 and RNF40 [PMID:22031019,21827756].