RNF6 Polyklonaler Antikörper

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• KD/KO Validated

RNF6 Polyklonal Antikörper für WB, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, IP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(7)

Kat-Nr. : 20437-1-AP

Synonyme

E3 ubiquitin-protein ligase RNF6, EC:2.3.2.27, SPG2

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  WB analysis using 20437-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 20437-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 20437-1-AP

  IP result of anti-RNF6 (IP:20437-1-AP, 4ug; Detection:20437-1-AP 1:800) with mouse testis tissue lysate 4000ug.

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  IF Staining of SW480 using 20437-1-AP

  Immunofluorescent analysis of (4% PFA) fixed SW480 cells using RNF6 antibody (20437-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: DU 145-Zellen, Maushodengewebe, PC-3-Zellen, SKOV-3-Zellen, Sp2/0-Zellen
• Erfolgreiche IP: Maushodengewebe
• Erfolgreiche Detektion in IF/ICC: SW480-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

20437-1-AP bindet in WB, IHC, IF/ICC, IP, ChIP, ELISA RNF6 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNF6 fusion protein Ag14209
• Vollständiger Name: ring finger protein (C3H2C3 type) 6
• Berechnetes Molekulargewicht: 685 aa, 78 kDa
• Beobachtetes Molekulargewicht: 78 kDa
• GenBank-Zugangsnummer: BC034688
• Gene symbol: RNF6
• Gene ID (NCBI): 6049
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF6 was firstly cloned in a genetic study of chromosomal rearrangements in myeloproliferative disorders, mapping to chromosome 13q12.12 [PMID:10331950]. It containing a coiled-coil domain at the N-terminus, and a RING-H2 finger at the C-terminus which is responsible for its ubiquitin ligase activity. It also activate Inha gene expression by participating in a protein complex binding to the promoter region [PMID:11971979]. In addition, LIMK1 is a substrate of RNF6 and the RNF6-induced LIMK1 polyubiquitination is mediated via K48 of ubiquitin and leads to proteasomal degradation of the kinase [PMID:16204183].

Publikationen

Species	Application	Title
mouse,human	WB,IF,ChIP	Oncogene; RING-finger protein 6 promotes colorectal tumorigenesis by transcriptionally activating SF3B2.; Authors - Hui Xu ; KO Validated
human	IHC	Cancer Lett; Ring finger protein 6 enhances chemo-resistance by transcriptionally activating proliferating cell nuclear antigen expression and attenuating DNA damage in lung adenocarcinoma.; Authors - Yile Sun
human	WB	Acta Pharmacol Sin; RNF6 promotes myeloma cell proliferation and survival by inducing glucocorticoid receptor polyubiquitination.; Authors - Ying Ren
human	WB	Anticancer Drugs; miR-203a suppresses cell proliferation by targeting RING-finger protein 6 in colorectal cancer.; Authors - Jiyu Miao
human	WB,IP	J Biol Chem; Induction of zinc finger protein RNF6 auto-ubiquitination for the treatment of myeloma and chronic myeloid leukemia; Authors - Haixia Zhuang
human	WB,IHC	Int J Biol Sci; TRIM26 promotes non-small cell lung cancer survival by inducing PBX1 degradation; Authors - Yuening Sun