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RNF6 Polyklonaler Antikörper

RNF6 Polyklonal Antikörper für WB, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 20437-1-PBS

Synonyme

E3 ubiquitin-protein ligase RNF6, EC:2.3.2.27, SPG2

Filter:
  • Western Blot (WB) analysis of various lysates using RNF6 Polyclonal antibody (20437-1-AP)

    WB analysis using 20437-1-AP (same clone as 20437-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse testis tissue using RNF6 Polyclonal antibody (20437-1-AP)

    WB analysis of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 20437-1-AP (RNF6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using RNF6 Polyclonal antibody (20437-1-AP)

    IP experiment of mouse testis using 20437-1-AP (same clone as 20437-1-PBS)

    IP result of anti-RNF6 (IP:20437-1-AP, 4ug; Detection:20437-1-AP 1:800) with mouse testis tissue lysate 4000ug. This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SW480 cells using RNF6 Polyclonal antibody (20437-1-AP)

    IF Staining of SW480 using 20437-1-AP (same clone as 20437-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SW480 cells using RNF6 antibody (20437-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4), CL488-phalloidin (green). This data was developed using the same antibody clone with 20437-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

20437-1-PBS bindet in WB, IF/ICC, IP, Indirect ELISA RNF6 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen RNF6 fusion protein Ag14209
Vollständiger Name ring finger protein (C3H2C3 type) 6
Berechnetes Molekulargewicht 685 aa, 78 kDa
Beobachtetes Molekulargewicht 78 kDa
GenBank-ZugangsnummerBC034688
Gene symbol RNF6
Gene ID (NCBI) 6049
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNF6 was firstly cloned in a genetic study of chromosomal rearrangements in myeloproliferative disorders, mapping to chromosome 13q12.12 [PMID:10331950]. It containing a coiled-coil domain at the N-terminus, and a RING-H2 finger at the C-terminus which is responsible for its ubiquitin ligase activity. It also activate Inha gene expression by participating in a protein complex binding to the promoter region [PMID:11971979]. In addition, LIMK1 is a substrate of RNF6 and the RNF6-induced LIMK1 polyubiquitination is mediated via K48 of ubiquitin and leads to proteasomal degradation of the kinase [PMID:16204183].