RNGTT Rekombinanter Antikörper

RNGTT Rekombinant Antikörper für WB, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240927A10

Kat-Nr. : 83974-2-PBS

Synonyme

240927A10, CAP1A, GTase, GTP  RNA guanylyltransferase, GTP--RNA guanylyltransferase

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  WB analysis using 83974-2-RR (same clone as 83974-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83974-2-RR (RNGTT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83974-2-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 83974-2-RR (same clone as 83974-2-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug RNGTT Recombinant antibody (83974-2-RR, Clone:240927A10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83974-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00899-2

  Cytometric bead array standard curve of MP00899-2, RNGTT Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83974-2-PBS. Detection antibody: 83974-3-PBS. Standard: Ag3105. Range: 0.781-100 ng/mL.

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  Affinity and Kinetic Characterization of 83974-2-PBS

  Biolayer interferometry (BLl) kinetic assay of 83974-2-PBS against Human RNGTT was performed. The affinity constant is 11.2 nM.

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  Affinity and Kinetic Characterization of 83974-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83974-2-RR against Human RNGTT were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

Produktinformation

83974-2-PBS bindet in WB, FC (Intra), Cytometric bead array, Indirect ELISA RNGTT und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RNGTT fusion protein Ag3105
• Vollständiger Name: RNA guanylyltransferase and 5'-phosphatase
• Berechnetes Molekulargewicht: 597 aa, 69 kDa
• Beobachtetes Molekulargewicht: 62-68 kDa
• GenBank-Zugangsnummer: BC019954
• Gene symbol: RNGTT
• Gene ID (NCBI): 8732
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNGTT(mRNA-capping enzyme) is also named as HCAP1, HCE, CAP1A. In the N-terminal section it belongs to the non-receptor class of the protein-tyrosine phosphatase family and in the C-terminal section it belongs to the eukaryotic GTase family. It is a bifunctional 597-amino acid protein with RNA triphosphatase and RNA guanylyltransferase activities(PMID:9545288). It has 4 isoforms produced by alternative splicing.