RNMTL1 Polyklonaler Antikörper

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RNMTL1 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 14707-1-AP

Synonyme

RMTL1, MRM3, HC90, EC:2.1.1.-, 16S rRNA [Gm1370] 2'-O-methyltransferase

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  WB analysis of HeLa using 14707-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14707-1-AP (RNMTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 14707-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14707-1-AP (RNMTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 14707-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14707-1-AP (RNMTL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 14707-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14707-1-AP (RNMTL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14707-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNMTL1 antibody (14707-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:5000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

14707-1-AP bindet in WB, IHC, IF/ICC, ELISA RNMTL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNMTL1 fusion protein Ag6399
• Vollständiger Name: RNA methyltransferase like 1
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC050614
• Gene symbol: RNMTL1
• Gene ID (NCBI): 55178
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The RNA methyltransferase like 1 gene (RNMTL1) is one of thirteen newly discovered genes within a 116 Kb segment of the chromosome 17p13.3, which suffers from a high frequent loss of heterozygosity in human hepatocellular carcinoma. Its amino acid sequence was identified a high conserved region with methylases from various microorganisms, suggesting to have function to transfer methyl group to rRNA molecule [PMID:12296377].

Publikationen

Species	Application	Title
human, mouse	WB,IF	J Biol Chem; Mitochondrial rRNA Methyltransferase Family Members are Positioned to Modify Nascent rRNA in Foci Near the mtDNA Nucleoid.; Authors - Lee Ken-Wing KW ; KD Validated
Human	IHC	PeerJ; REG γ knockdown suppresses proliferation by inducing apoptosis and cell cycle arrest in osteosarcoma.; Authors - Zhiqiang Yin