RNMTL1 Polyklonaler Antikörper

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RNMTL1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 14707-1-PBS

Synonyme

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  WB analysis of HeLa using 14707-1-AP (same clone as 14707-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14707-1-AP (RNMTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 14707-1-AP (same clone as 14707-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14707-1-AP (RNMTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 14707-1-AP (same clone as 14707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14707-1-AP (RNMTL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 14707-1-AP (same clone as 14707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14707-1-AP (RNMTL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14707-1-AP (same clone as 14707-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNMTL1 antibody (14707-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

14707-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RNMTL1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RNMTL1 fusion protein Ag6399
• Vollständiger Name: RNA methyltransferase like 1
• Berechnetes Molekulargewicht: 47 kDa
• Beobachtetes Molekulargewicht: 47 kDa
• GenBank-Zugangsnummer: BC050614
• Gene symbol: RNMTL1
• Gene ID (NCBI): 55178
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The RNA methyltransferase like 1 gene (RNMTL1) is one of thirteen newly discovered genes within a 116 Kb segment of the chromosome 17p13.3, which suffers from a high frequent loss of heterozygosity in human hepatocellular carcinoma. Its amino acid sequence was identified a high conserved region with methylases from various microorganisms, suggesting to have function to transfer methyl group to rRNA molecule [PMID:12296377].