ROBO1 Monoklonaler Antikörper

ROBO1 Monoklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

2H4H5

Kat-Nr. : 67922-1-PBS

Synonyme

2H4H5, Deleted in U twenty twenty, DUTT1, H Robo 1, H-Robo-1

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  WB analysis of Y79 using 67922-1-Ig (same clone as 67922-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 67922-1-Ig (ROBO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67922-1-PBS in a different storage buffer formulation.

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  WB analysis of U-87 MG using 67922-1-Ig (same clone as 67922-1-PBS)

  U-87 MG cells were subjected to SDS PAGE followed by western blot with 67922-1-Ig (ROBO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67922-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 67922-1-Ig (same clone as 67922-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67922-1-Ig (ROBO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67922-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 67922-1-Ig (same clone as 67922-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67922-1-Ig (ROBO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67922-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67922-1-Ig (same clone as 67922-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67922-1-Ig (ROBO1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67922-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67922-1-Ig (same clone as 67922-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67922-1-Ig (ROBO1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67922-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67922-1-Ig (same clone as 67922-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67922-1-Ig (ROBO1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67922-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67922-1-Ig (same clone as 67922-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67922-1-Ig (ROBO1 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67922-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse cerebellum using 67922-1-Ig (same clone as 67922-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67922-1-Ig (ROBO1 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67922-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67922-1-PBS bindet in WB, IHC, ELISA ROBO1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ROBO1 fusion protein Ag18074
• Vollständiger Name: roundabout, axon guidance receptor, homolog 1 (Drosophila)
• Berechnetes Molekulargewicht: 1651 aa, 181 kDa
• Beobachtetes Molekulargewicht: 200-250 kDa
• GenBank-Zugangsnummer: BC112336
• Gene symbol: ROBO1
• Gene ID (NCBI): 6091
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ROBO1, also named as DUTT1, belongs to the immunoglobulin superfamily and ROBO family. It is a receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. It may be required for lung development.