ROBO3-Specific Polyklonaler Antikörper

ROBO3-Specific Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(2)

Kat-Nr. : 20220-1-AP

Synonyme

ROBO3, HGPPS, HGPS, RBIG1, RIG1

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  WB analysis using 20220-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20220-1-AP (ROBO3-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human gliomas using 20220-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas slide using 20220-1-AP (ROBO3-Specific Antibody) at dilution of 1:50.

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  IHC staining of mouse brain using 20220-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20220-1-AP (ROBO3-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20220-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20220-1-AP (ROBO3-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human brain using 20220-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 20220-1-AP (ROBO3-Specific Antibody) at dilution of 1:50.

• 

  IHC staining of human brain using 20220-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain slide using 20220-1-AP (ROBO3-Specific Antibody) at dilution of 1:50.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Neuro-2a-Zellen, Maushirngewebe, Rattenhirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe, humanes Gliomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

20220-1-AP bindet in WB, IHC, IF, ELISA ROBO3-Specific und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: roundabout, axon guidance receptor, homolog 3 (Drosophila)
• Berechnetes Molekulargewicht: 148 kDa
• Beobachtetes Molekulargewicht: 148 kDa
• GenBank-Zugangsnummer: NM_022370
• Gene symbol: ROBO3
• Gene ID (NCBI): 64221
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ROBO3 belongs to the immunoglobulin superfamily and ROBO family. ROBO3 is thought to be involved during neural development in axonal navigation at the ventral midline of the neural tube. In spinal cord development, it plays a role in guiding commissural axons probably by preventing premature sensitivity to Slit proteins thus inhibiting Slit signaling through ROBO1. It is required for hindbrain axon midline crossing. Defects in ROBO3 are a cause of familial horizontal gaze palsy with progressive scoliosis (HGPPS). Patients show a medulla where motor and sensory projections appear uncrossed. The antibody is specific to ROBO3.

Publikationen

Species	Application	Title
human	IF	J Biol Chem; Non-redundant roles of DIAPHs in primary ciliogenesis.; Authors - Oliva Palander
mouse	WB	Brain Res Bull; CircSATB2 Modulates Fear Extinction Memory via Robo3-Driven Synaptic Plasticity; Authors - Ziyue Xu