ROCK1 Polyklonaler Antikörper

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• KD/KO Validated

ROCK1 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 21850-1-PBS

Synonyme

Rho-associated protein kinase 1, Renal carcinoma antigen NY-REN-35, P160ROCK, p160 ROCK-1, p160 ROCK 1

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  WB analysis using 21850-1-AP (same clone as 21850-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 21850-1-AP (ROCK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 21850-1-AP (same clone as 21850-1-PBS)

  WB result of ROCK1 antibody (21850-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK1 transfected HeLa cells. This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 21850-1-AP (same clone as 21850-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21850-1-AP (ROCK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 21850-1-AP (same clone as 21850-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 21850-1-AP (ROCK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 21850-1-AP (same clone as 21850-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ROCK1 antibody (21850-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21850-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

21850-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA ROCK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ROCK1 fusion protein Ag16317
• Vollständiger Name: Rho-associated, coiled-coil containing protein kinase 1
• Berechnetes Molekulargewicht: 1354 aa, 158 kDa
• Beobachtetes Molekulargewicht: 150-160 kDa
• GenBank-Zugangsnummer: BC113114
• Gene symbol: ROCK1
• Gene ID (NCBI): 6093
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.