• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

ROCK1 Monoklonaler Antikörper

ROCK1 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

3G5E12

Kat-Nr. : 66782-1-PBS

Synonyme

3G5E12, EC:2.7.11.1, p160 ROCK 1, p160 ROCK-1, P160ROCK

Filter:
  • Western Blot (WB) analysis of various lysates using ROCK1 Monoclonal antibody (66782-1-Ig)

    WB analysis using 66782-1-Ig (same clone as 66782-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66782-1-Ig (ROCK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66782-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ROCK1 Monoclonal antibody (66782-1-Ig)

    WB analysis of HeLa using 66782-1-Ig (same clone as 66782-1-PBS)

    WB result of ROCK1 antibody (66782-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK1 transfected HeLa cells. This data was developed using the same antibody clone with 66782-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66782-1-PBS bindet in WB, Indirect ELISA ROCK1 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen ROCK1 fusion protein Ag16317
Vollständiger Name Rho-associated, coiled-coil containing protein kinase 1
Berechnetes Molekulargewicht 1354 aa, 158 kDa
Beobachtetes Molekulargewicht 150-160 kDa
GenBank-ZugangsnummerBC113114
Gene symbol ROCK1
Gene ID (NCBI) 6093
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ROCK1, also named as p160ROCK and NY-REN-35, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. ROCK1 phosphorylates and activates DAPK3, which then regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. ROCK1 is required for centromere positioning and centromere-dependent exit from mitosis. It is necessary for apoptotic membrane blebbing. ROCK1 catalyzes the reaction: ATP + a protein = ADP + a phosphoprotein.