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ROCK2 Monoklonaler Antikörper

ROCK2 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C7B8

Kat-Nr. : 66633-1-PBS

Synonyme

1C7B8, EC:2.7.11.1, KIAA0619, p164 ROCK-2, Rho kinase 2

Filter:
  • Western Blot (WB) analysis of various lysates using ROCK2 Monoclonal antibody (66633-1-Ig)

    WB analysis using 66633-1-Ig (same clone as 66633-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66633-1-Ig (ROCK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using ROCK2 Monoclonal antibody (66633-1-Ig)

    WB analysis of HeLa using 66633-1-Ig (same clone as 66633-1-PBS)

    WB result of ROCK2 antibody (66633-1-Ig; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROCK2 transfected HeLa cells. This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ROCK2 Monoclonal antibody (66633-1-Ig)

    IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66633-1-Ig (ROCK2 antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using ROCK2 Monoclonal antibody (66633-1-Ig)

    IHC staining of human liver cancer using 66633-1-Ig (same clone as 66633-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66633-1-Ig (ROCK2 antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66633-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

66633-1-PBS bindet in WB, IHC, Indirect ELISA ROCK2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Maus / IgG2b
Klonalität Monoklonal
Typ Antikörper
Immunogen ROCK2 fusion protein Ag16330
Vollständiger Name Rho-associated, coiled-coil containing protein kinase 2
Berechnetes Molekulargewicht 77 kDa, 161 kDa
Beobachtetes Molekulargewicht 161 kDa
GenBank-ZugangsnummerBC111801
Gene symbol ROCK2
Gene ID (NCBI) 9475
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ROCK2 is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. It is an isozyme of ROCK1, is a target for the small GTPase Rho. ROCK2 is localized in both cytoplasm and nucleus, and the nucleus-localized ROCK2 can target p300 for phosphorylation to regulate its acetyltransferase activity (PMID:16574662).This antibody is specific to ROCK2.