RON, MST1R Monoklonaler Antikörper

RON, MST1R Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

2D8D1

Kat-Nr. : 67954-1-Ig

Synonyme

MST1R, Macrophage-stimulating protein receptor alpha chain, Macrophage-stimulating protein receptor, EC:2.7.10.1, CDw136

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  WB analysis of BxPC-3 using 67954-1-Ig

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 67954-1-Ig (RON, MST1R antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67954-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67954-1-Ig (RON, MST1R antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 67954-1-Ig

  PC-3 cells were subjected to SDS PAGE followed by western blot with 67954-1-Ig (RON, MST1R antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 67954-1-Ig

  1x10^6 HeLa cells were intracellularly stained with 0.4 ug RON, MST1R Monoclonal antibody (67954-1-Ig, Clone:2D8D1) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: BxPC-3-Zellen, HeLa-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in FC (Intra): HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Durchflusszytometrie (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

67954-1-Ig bindet in WB, ELISA RON, MST1R und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RON, MST1R fusion protein Ag28289
• Vollständiger Name: macrophage stimulating 1 receptor (c-met-related tyrosine kinase)
• Berechnetes Molekulargewicht: 152 kDa
• Beobachtetes Molekulargewicht: 150-185 kDa
• GenBank-Zugangsnummer: BQ936677 NM_002447
• Gene symbol: RON
• Gene ID (NCBI): 4486
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.