ROR1 Monoklonaler Antikörper

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• KD/KO Validated

ROR1 Monoklonal Antikörper für WB, IHC, Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F5D8

Kat-Nr. : 66923-1-PBS

Synonyme

1F5D8, Inactive tyrosine-protein kinase transmembrane receptor ROR1, Neurotrophic tyrosine kinase, receptor-related 1, NTRKR1

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  WB analysis using 66923-1-Ig (same clone as 66923-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 66923-1-PBS in a different storage buffer formulation.

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  WB analysis using 66923-1-Ig (same clone as 66923-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66923-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 66923-1-Ig (same clone as 66923-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66923-1-PBS in a different storage buffer formulation.

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  WB analysis of NIH/3T3 using 66923-1-Ig (same clone as 66923-1-PBS)

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 66923-1-Ig (ROR1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66923-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66923-1-Ig (same clone as 66923-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66923-1-Ig (ROR1 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66923-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66923-1-Ig (same clone as 66923-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66923-1-Ig (ROR1 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66923-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51139-1

  Cytometric bead array standard curve of MP51139-1, ROR1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66923-2-PBS. Detection antibody: 66923-1-PBS. Standard:Ag14586. Range: 0.391-100 ng/mL.

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  Cytometric bead array standard curve of MP51139-2

  Cytometric bead array standard curve of MP51139-2, ROR1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66923-3-PBS. Detection antibody: 66923-1-PBS. Standard:Ag14586. Range: 0.781-100 ng/mL.

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Geprüfte Anwendungen

Produktinformation

66923-1-PBS bindet in WB, IHC, Cytometric bead array, Indirect ELISA ROR1 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: ROR1 fusion protein Ag14586
• Vollständiger Name: receptor tyrosine kinase-like orphan receptor 1
• Berechnetes Molekulargewicht: 937 aa, 104 kDa
• Beobachtetes Molekulargewicht: 130 kDa
• GenBank-Zugangsnummer: BC006374
• Gene symbol: ROR1
• Gene ID (NCBI): 4919
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ROR1 is a member of the RTK family of orphan receptors related to muscle-specific kinase and Trk neurotrophin receptors (PMID: 18546292). ROR1 is primarily expressed by neural crest cells during embryogenesis. High expression of ROR1 is reported in many types of malignancies and is thought to be involved in tumor growth, apoptosis, and epithelial-mesenchymal transition (PMID: 26245996). The human ROR1 gene encodes a 937 amino acid protein with an Ig-like domain, a cysteine-rich domain, a kringle domain, a tyrosine kinase domain, and a proline-rich domain. The calculated molecular weight of ROR1 is 104 kDa, but ROR1 has multiple N-glycosylation sites that generate post-translationally modified ROR1 at 130 kDa (PMID: 24752542).