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ROR1 Monoklonaler Antikörper

ROR1 Monoklonal Antikörper für FC (Intra)

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

FC (Intra)

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

1F5D8

Kat-Nr. : CL594-66923

Synonyme

dJ537F10.1, NTRKR1, ROR1

Galerie der Validierungsdaten

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  • Flow cytometry (FC) experiment of K-562 cells using CoraLite®594-conjugated ROR1 Monoclonal antibody (CL594-66923)

    FC experiment of K-562 using CL594-66923

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human ROR1 (CL594-66923, Clone:1F5D8) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Geprüfte Anwendungen

Erfolgreiche Detektion in FCK-562-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-66923 bindet in FC (Intra) ROR1 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen ROR1 fusion protein Ag14586
Vollständiger Name receptor tyrosine kinase-like orphan receptor 1
Berechnetes Molekulargewicht 937 aa, 104 kDa
GenBank-ZugangsnummerBC006374
Gene symbol ROR1
Gene ID (NCBI) 4919
Konjugation CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
LagerungsbedingungenBei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ROR1 is a member of the RTK family of orphan receptors related to muscle specific kinase and Trk neurotrophin receptors (PMID: 18546292). ROR1 is primarily expressed by neural crest cells during embryogenesis. High expression of ROR1 is reported in many types of malignancies and is thought to be involved in tumor growth, apoptosis, and epithelial-mesenchymal transition (PMID: 26245996). The human ROR1 gene encodes a 937-amino acid protein with an Ig-like domain, a cysteine-rich domain, a kringle domain, a tyrosine kinase domain and a proline-rich domain. The calculated molecular weight of ROR1 is 104 kDa, but ROR1 has multiple N-glycosylation sites that generate post-translationally modified ROR1 at 130 kDa (PMID: 24752542).

Protokolle

Produktspezifische Protokolle
FC protocol for CL594 ROR1 antibody CL594-66923Protokoll herunterladen
Standard-Protokolle
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