RORA Polyklonaler Antikörper

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RORA Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 10616-1-PBS

Synonyme

NR1F1, Nuclear receptor ROR-alpha, Nuclear receptor RZR-alpha, RAR-related orphan receptor A, Retinoid-related orphan receptor-alpha

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  WB analysis using 10616-1-AP (same clone as 10616-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10616-1-PBS in a different storage buffer formulation.

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  WB analysis of PC-3 using 10616-1-AP (same clone as 10616-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10616-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 10616-1-AP (same clone as 10616-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10616-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 10616-1-AP (same clone as 10616-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10616-1-PBS in a different storage buffer formulation.

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  WB analysis of Jurkat using 10616-1-AP (same clone as 10616-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10616-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

10616-1-PBS bindet in WB, ELISA RORA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RORA fusion protein Ag0944
• Vollständiger Name: RAR-related orphan receptor A
• Berechnetes Molekulargewicht: 59 kDa
• Beobachtetes Molekulargewicht: 65-70 kDa
• GenBank-Zugangsnummer: BC008831
• Gene symbol: RORA
• Gene ID (NCBI): 6095
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RORA is a steroid hormone receptor and has been implicated in the pathology of circadian rhythms, bone growth, angiogenesis, development of cones, cellular metabolism and a mediator in the immune and lipid metabolism pathways [PMID:21998696]. It binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence, which binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPAR gamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM and ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1 [PMID:11053433,14570920].