RORB Polyklonaler Antikörper

RORB Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

3

Rezensionen (1)

Kat-Nr. : 17635-1-AP

Synonyme

NR1F2, Nuclear receptor ROR beta, Nuclear receptor RZR beta, RAR related orphan receptor B, ROR BETA, RORB, RZR BETA, RZRB

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Galerie der Validierungsdaten

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  WB analysis of Y79 using 17635-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17635-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17635-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17635-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 17635-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 17635-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 17635-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 17635-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Y79 using 17635-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17635-1-AP (RORB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 17635-1-AP

  IP Result of anti-RORB (IP:17635-1-AP, 4ug; Detection:17635-1-AP 1:300) with mouse brain tissue lysate 3440ug.

• 

  IHC staining of mouse brain using 17635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 17635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of mouse brain using 17635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of mouse brain using 17635-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17635-1-AP (RORB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, Maushirngewebe, Rattenhirngewebe, SH-SY5Y-Zellen
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 3 publications below

Produktinformation

17635-1-AP bindet in WB, IP, IHC, IF, ELISA RORB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RORB fusion protein Ag11910
• Vollständiger Name: RAR-related orphan receptor B
• Berechnetes Molekulargewicht: 459 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC051830
• Gene symbol: RORB
• Gene ID (NCBI): 6096
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear receptors (NRs) are a large group of ligand-inducible transcription factors that regulate many facets of mammalian Physiology [PMID:22189870]. In addition, NRs that lack identified ligands and are termed orphan receptors. RORB is a transcription factor and belongs to the nuclear receptor family [PMID:7935491], which share a common modular structure composed of a transactivation domain, a DNA-binding domain, and a ligand-binding. Its function as transcriptional factor is regulated by small lipophilic molecules, such as steroid hormones, vitamin D, retinoic acids, and thyroid hormone [PMID:17431400].

Publikationen

Species	Application	Title
mouse	IF	Neuron; Selective removal of astrocytic APOE4 strongly protects against tau-mediated neurodegeneration and decreases synaptic phagocytosis by microglia.; Authors - Chao Wang
mouse	IF	PLoS Biol; An adhesion signaling axis involving Dystroglycan, β1-Integrin, and Cas adaptor proteins regulates the establishment of the cortical glial scaffold; Authors - Wenny Wong
mouse	IF	eNeuro; Cellular and Behavioral Characterization of Pcdh19 Mutant Mice: subtle Molecular Changes, Increased Exploratory Behavior and an Impact of Social Environment.; Authors - Natalia Galindo-Riera

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Etienne (Verified Customer) (12-19-2022)

Did not work at all in human brain organoids (where the target is present)

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/50, 1/200, 1/500
• Cell Tissue Type: Human Brain Organoids

RORB Antibody Immunofluorescence validation (1/50, 1/200, 1/500 dilution) in Human Brain Organoids (Cat no:17635-1-AP)