RORB Rekombinanter Antikörper

RORB Rekombinant Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

242832D9

Kat-Nr. : 85404-4-RR

Synonyme

NR1F2, Nuclear receptor ROR-beta, Nuclear receptor RZR-beta, Retinoid-related orphan receptor-beta, RZRB

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  WB analysis using 85404-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85404-4-RR (RORB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse eye using 85404-4-RR

  IP result of anti-RORB (IP:85404-4-RR, 4ug; Detection:85404-4-RR 1:10000) with mouse eye tissue lysate 2680 ug.

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  Affinity and Kinetic Characterization of 85404-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85404-4-RR against Human RORB were performed. The affinity constant is 0.412 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Retina-Gewebe, Ratten-Retina-Gewebe
• Erfolgreiche IP: Maus-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:15000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85404-4-RR bindet in WB, IP, ELISA RORB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: RORB fusion protein Ag11910
• Vollständiger Name: RAR-related orphan receptor B
• Berechnetes Molekulargewicht: 459 aa, 52 kDa
• Beobachtetes Molekulargewicht: 52 kDa
• GenBank-Zugangsnummer: BC051830
• Gene symbol: RORB
• Gene ID (NCBI): 6096
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Nuclear receptors (NRs) are a large group of ligand-inducible transcription factors that regulate many facets of mammalian Physiology (PMID: 22189870). In addition, NRs that lack identified ligands are termed orphan receptors. RORB is a transcription factor and belongs to the nuclear receptor family (PMID: 7935491), which shares a common modular structure composed of a transactivation domain, a DNA-binding domain, and a ligand-binding. Its function as the transcriptional factor is regulated by small lipophilic molecules, such as steroid hormones, vitamin D, retinoic acids, and thyroid hormone (PMID: 17431400).