MST4 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

MST4 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(9)

Rezensionen (1)

Kat-Nr. : 10847-1-AP

Synonyme

STK26, MST3 and SOK1-related kinase, Mst3 and SOK1 related kinase, MST 4, MASK

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• 

  WB analysis of MCF-7 using 10847-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10847-1-AP (RP6-213H19.1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10847-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10847-1-AP (MST4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 10847-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 10847-1-AP (RP6-213H19.1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 10847-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10847-1-AP (RP6-213H19.1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of MCF-7 using 10847-1-AP

  IP result of anti-MST4 (IP:10847-1-AP, 4ug; Detection:10847-1-AP 1:500) with MCF-7 cells lysate 2400ug.

• 

  IHC staining of human liver using 10847-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10847-1-AP (MST4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 10847-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10847-1-AP (MST4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10847-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 10847-1-AP (RP6-213H19.1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of MCF-7 using 10847-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using MST4 antibody (10847-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HL-60-Zellen, Jurkat-Zellen, L02-Zellen, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Lebergewebe, humanes Kolonkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 4 publications below
• IF: See 4 publications below
• IP: See 1 publications below

Produktinformation

10847-1-AP bindet in WB, IHC, IF/ICC, IP, ELISA MST4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: MST4 fusion protein Ag1302
• Vollständiger Name: serine/threonine protein kinase MST4
• Berechnetes Molekulargewicht: 16 kDa, 46 kDa
• Beobachtetes Molekulargewicht: 47-50 kDa
• GenBank-Zugangsnummer: BC017213
• Gene symbol: MST4
• Gene ID (NCBI): 51765
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB,IF	J Cell Sci; CLIC4 is a cytokinetic cleavage furrow protein that regulates cortical cytoskeleton stability during cell division.; Authors - Eric Peterman ; KD Validated
human	WB,IHC,IF	Int J Oncol; MST4 promotes hepatocellular carcinoma epithelial-mesenchymal transition and metastasis via activation of the p-ERK pathway.; Authors - Zhen-Hai Lin ; KD Validated
mouse	WB,IF	Brain Res Bull; MST4 attenuates NLRP3 inflammasome-mediated neuroinflammation and affects the prognosis after intracerebral hemorrhage in mice.; Authors - Xiaodong Wu
human	IHC	Onco Targets Ther; MST4 Regulates Epithelial-Mesenchymal Transition of Choriocarcinoma by Mediating TGF-β1 Expression.; Authors - Hanxi Yu
mouse	WB,IF	Behav Neurol; MST4 Kinase Inhibitor Hesperadin Attenuates Autophagy and Behavioral Disorder via the MST4/AKT Pathway in Intracerebral Hemorrhage Mice.; Authors - Xiaodong Wu
human	WB	Antiviral Res; Chromatin binding protein HMGN1 promotes HBV cccDNA transcription and replication by regulating the phosphorylation of histone 3; Authors - Tan Ming

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Guillaume (Verified Customer) (07-21-2022)

Antibody worked well, band present at 55kDa

• Applications: Western Blot
• Cell Tissue Type: Mice liver tumor/ Mice healthy liver

MST4 Antibody Western Blot validation ( dilution) in Mice liver tumor/ Mice healthy liver (Cat no:10847-1-AP)