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POLR1A Rekombinanter Antikörper

POLR1A Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

243082E3

Kat-Nr. : 85633-1-PBS

Synonyme

A190, DNA-directed RNA polymerase I largest subunit, DNA-directed RNA polymerase I subunit A, DNA-directed RNA polymerase I subunit RPA1, EC:2.7.7.6

Filter:
  • Western Blot (WB) analysis of various lysates using POLR1A Recombinant antibody (85633-1-RR)

    WB analysis using 85633-1-RR (same clone as 85633-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85633-1-RR (POLR1A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85633-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85633-1-RR

    Affinity and Kinetic Characterization of 85633-1-RR

    Biolayer interferometry (BLl) kinetic assays of 85633-1-RR against Human POLR1A were performed. The affinity constant is below 1 pM.

Geprüfte Anwendungen

Produktinformation

85633-1-PBS bindet in WB, Indirect ELISA POLR1A und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen POLR1A fusion protein Ag14543
Vollständiger Name polymerase (RNA) I polypeptide A, 194kDa
Berechnetes Molekulargewicht 1720 aa, 195 kDa
Beobachtetes Molekulargewicht190-195 kDa
GenBank-ZugangsnummerBC017115
Gene symbol POLR1A
Gene ID (NCBI) 25885
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

POLR1A, DNA-directed RNA polymerase I subunit RPA1, is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA. RNA polymerase I (Pol I) located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. Different subunits of the Pol I transcription are targets of various physiological stimuli, which suggests that multiple signaling pathways are involved in carrying out Pol I ranscription. Together with the second largest subunit, it is the catalytic core component of RNA polymerase I that synthesizes ribosomal RNA precursors.