RPA2 Monoklonaler Antikörper

RPA2 Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Kaninchen, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1B9H11

Kat-Nr. : 67999-1-PBS

Synonyme

RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2, REPA2

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  WB analysis using 67999-1-Ig (same clone as 67999-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

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  WB analysis of pig spleen using 67999-1-Ig (same clone as 67999-1-PBS)

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

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  WB analysis of rat testis using 67999-1-Ig (same clone as 67999-1-PBS)

  rat testis tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 67999-1-Ig (same clone as 67999-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rabbit spleen using 67999-1-Ig (same clone as 67999-1-PBS)

  rabbit spleen tissue were subjected to SDS PAGE followed by western blot with 67999-1-Ig (RPA2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67999-1-Ig (same clone as 67999-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (67999-1-Ig, Clone: 1B9H11 ) at dilution of 1:4000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), (CL594-Phalloidin, red). This data was developed using the same antibody clone with 67999-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

67999-1-PBS bindet in WB, IF/ICC, Indirect ELISA RPA2 und zeigt Reaktivität mit Hausschwein, human, Kaninchen, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Kaninchen, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RPA2 fusion protein Ag24783
• Vollständiger Name: replication protein A2, 32kDa
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC001630
• Gene symbol: RPA2
• Gene ID (NCBI): 6118
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Thr residues that are phosphorylated in sequential order in response to genotoxic stress.