RPAIN Polyklonaler Antikörper

RPAIN Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 15220-1-PBS

Synonyme

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  WB analysis of A2780 using 15220-1-AP (same clone as 15220-1-PBS)

  A2780 cells were subjected to SDS PAGE followed by western blot with 15220-1-AP (RPAIN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15220-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 15220-1-AP (same clone as 15220-1-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 15220-1-AP (RPAIN Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15220-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse ovary using 15220-1-AP (same clone as 15220-1-PBS)

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 15220-1-AP (RPAIN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15220-1-PBS in a different storage buffer formulation.

• 

  IP experiment of A375 using 15220-1-AP (same clone as 15220-1-PBS)

  IP result of anti-RPAIN (IP:15220-1-AP, 4ug; Detection:15220-1-AP 1:300) with A375 cells lysate 2200 ug. This data was developed using the same antibody clone with 15220-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary tumor using 15220-1-AP (same clone as 15220-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15220-1-AP (RPAIN antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 15220-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human ovary tumor using 15220-1-AP (same clone as 15220-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15220-1-AP (RPAIN antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 15220-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

15220-1-PBS bindet in WB, IHC, IP, Indirect ELISA RPAIN und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPAIN fusion protein Ag7392
• Vollständiger Name: RPA interacting protein
• Berechnetes Molekulargewicht: 25 kDa
• Beobachtetes Molekulargewicht: 30-45 kDa
• GenBank-Zugangsnummer: BC004451
• Gene symbol: RPAIN
• Gene ID (NCBI): 84268
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PAIN, also named as RIP, mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. RPAIN has many isoforms with MW 25-27 kDa, 12 kDa and 16-19 kDa. The publication with PMID: 16135809 reported the MW 30 and 45 kDa.