RPAP1 Monoklonaler Antikörper

RPAP1 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

2A6H5

Kat-Nr. : 68193-1-PBS

Synonyme

RPAP 1, RNA polymerase II-associated protein 1, KIAA1403, 2A6H5

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  WB analysis using 68193-1-Ig (same clone as 68193-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68193-1-Ig (RPAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68193-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 68193-1-Ig (same clone as 68193-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 68193-1-Ig (RPAP1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68193-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 68193-1-Ig (same clone as 68193-1-PBS)

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug RPAP1 Monoclonal antibody (68193-1-Ig, Clone:2A6H5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.8 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 68193-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

68193-1-PBS bindet in WB, Indirect ELISA RPAP1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: RPAP1 fusion protein Ag7856
• Vollständiger Name: RNA polymerase II associated protein 1
• Berechnetes Molekulargewicht: 153 kDa
• Beobachtetes Molekulargewicht: 153 kDa
• GenBank-Zugangsnummer: BC000246
• Gene symbol: RPAP1
• Gene ID (NCBI): 26015
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RNA polymerase II-associated protein 1 (RPAP1) encodes a nuclear 153-kDa protein, and forms an interface between the RNA polymerase II (RNAPII) enzyme and chaperone/scaffolding protein, suggesting that it is required to connect RNA polymerase II to regulators of protein complex formation. RPAP1 is required for interaction of the RNA polymerase II complex with acetylated histone H3 and the subsequent DNA transcription.