RPAP3 Polyklonaler Antikörper

RPAP3 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 23741-1-PBS

Synonyme

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  WB analysis of HEK-293 using 23741-1-AP (same clone as 23741-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 23741-1-AP (same clone as 23741-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 23741-1-AP (same clone as 23741-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 23741-1-AP (same clone as 23741-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HEK-293 using 23741-1-AP (same clone as 23741-1-PBS)

  IP result of anti-RPAP3 (IP:23741-1-AP, 4ug; Detection:23741-1-AP 1:500) with HEK-293 cells lysate 2800ug. This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 23741-1-AP (same clone as 23741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23741-1-AP (RPAP3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human stomach cancer using 23741-1-AP (same clone as 23741-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23741-1-AP (RPAP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

• 

  IF Staining of HeLa using 23741-1-AP (same clone as 23741-1-PBS)

  Immunofluorescent analysis of HeLa cells using 23741-1-AP (RPAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 23741-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

23741-1-PBS bindet in WB, IHC, IF/ICC, IP, Indirect ELISA RPAP3 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPAP3 fusion protein Ag20661
• Vollständiger Name: RNA polymerase II associated protein 3
• Berechnetes Molekulargewicht: 76 kDa
• Beobachtetes Molekulargewicht: 76 kDa
• GenBank-Zugangsnummer: BC056415
• Gene symbol: RPAP3
• Gene ID (NCBI): 79657
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-affinitätsgereinigt
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.