RPE65 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

RPE65 Polyklonal Antikörper für WB, IHC, IF-P, IF-Fro, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte und mehr (1)

Anwendung

WB, IHC, IF-P, IF-Fro, ELISA

Konjugation

Unkonjugiert

Publikationen(18)

Rezensionen (1)

Kat-Nr. : 17939-1-AP

Synonyme

All-trans-retinyl-palmitate hydrolase, EC:3.1.1.64, EC:5.3.3.22, LCA2, Lutein isomerase

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  WB analysis of Y79 using 17939-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 17939-1-AP (RPE65 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 17939-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17939-1-AP (RPE65 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17939-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17939-1-AP (RPE65 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse eye using 17939-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 17939-1-AP (RPE65 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 17939-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 17939-1-AP (RPE65 antibody) at dilution of 1:200 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse eye using 17939-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using RPE65 antibody (17939-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse eye using 17939-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using RPE65 antibody (17939-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse eye using 17939-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse eye tissue using RPE65 antibody (17939-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Y79-Zellen, HeLa-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IHC: Maus-Augengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Augengewebe
• Erfolgreiche Detektion in IF-Fro: Maus-Augengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500
• Immunfluoreszenz (IF)-FRO: IF-FRO : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 8 publications below

Produktinformation

17939-1-AP bindet in WB, IHC, IF-P, IF-Fro, ELISA RPE65 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPE65 fusion protein Ag12356
• Vollständiger Name: retinal pigment epithelium-specific protein 65kDa
• Berechnetes Molekulargewicht: 61 kDa
• Beobachtetes Molekulargewicht: 65 kDa, 61 kDa
• GenBank-Zugangsnummer: BC075036
• Gene symbol: RPE65
• Gene ID (NCBI): 6121
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPE65(Retinal pigment epithelium-specific 65 kDa protein) is also named as retinoid isomerohydrolase with the calculated molecular weight og 61 kDa and belongs to the carotenoid oxygenase family. It is a microsomal protein with isomerase activity in the retinoid visual cycle that playing a role in the isomerisation of all-trans (vitamin A) to 11-cis retinal, the chromophore of the visual pigments. It also might be involved in retinoid uptake of keratinocytes.

Publikationen

Species	Application	Title
human	WB	Redox Biol; Glia maturation factor-β induces ferroptosis by impairing chaperone-mediated autophagic degradation of ACSL4 in early diabetic retinopathy.; Authors - Caiying Liu
	WB	J Biol Chem; Conversion of all-trans-retinal into all-trans-retinal dimer reflects an alternative metabolic/antidotal pathway of all-trans-retinal in the retina.; Authors - Zhan Gao
mouse	IF	FASEB J; Prph2 disease mutations lead to structural and functional defects in the RPE.; Authors - Lars Tebbe
rat	IF	Invest Ophthalmol Vis Sci; Dasatinib Plus Quercetin Alleviates Choroid Neovascularization by Reducing the Cellular Senescence Burden in the RPE-Choroid; Authors - Yinhao Wang
mouse	WB	Diabetes Res Clin Pract; Thioredoxin upregulation delays diabetes-induced photoreceptor cell degeneration via AMPK-mediated autophagy and exosome secretion.; Authors - Xiang Ren
rat	WB	Am J Pathol; Impaired Rhodopsin Generation in the Rat Model of Diabetic Retinopathy.; Authors - Volha V Malechka

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (11-06-2022)

no unspecific staining, great outcome

• Applications: Immunofluorescence