RPE65 Rekombinanter Antikörper
RPE65 Rekombinant Antikörper für WB, ELISA
Wirt / Isotyp
Kaninchen / IgG
Getestete Reaktivität
human
Anwendung
WB, ELISA
Konjugation
Unkonjugiert
CloneNo.
240518F1
Kat-Nr. : 83861-1-RR
Synonyme
Geprüfte Anwendungen
| Erfolgreiche Detektion in WB | HeLa-Zellen, PC-3-Zellen, Y79-Zellen |
Empfohlene Verdünnung
| Anwendung | Verdünnung |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Produktinformation
83861-1-RR bindet in WB, ELISA RPE65 und zeigt Reaktivität mit human
| Getestete Reaktivität | human |
| Wirt / Isotyp | Kaninchen / IgG |
| Klonalität | Rekombinant |
| Typ | Antikörper |
| Immunogen | RPE65 fusion protein Ag12356 |
| Vollständiger Name | retinal pigment epithelium-specific protein 65kDa |
| Berechnetes Molekulargewicht | 61 kDa |
| Beobachtetes Molekulargewicht | 65 kDa, 61 kDa |
| GenBank-Zugangsnummer | BC075036 |
| Gene symbol | RPE65 |
| Gene ID (NCBI) | 6121 |
| Konjugation | Unkonjugiert |
| Form | Liquid |
| Reinigungsmethode | Protein A purfication |
| Lagerungspuffer | PBS with 0.02% sodium azide and 50% glycerol |
| Lagerungsbedingungen | Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA. |
Hintergrundinformationen
RPE65(Retinal pigment epithelium-specific 65 kDa protein) is also named as retinoid isomerohydrolase with the calculated molecular weight og 61 kDa and belongs to the carotenoid oxygenase family. It is a microsomal protein with isomerase activity in the retinoid visual cycle that playing a role in the isomerisation of all-trans (vitamin A) to 11-cis retinal, the chromophore of the visual pigments. It also might be involved in retinoid uptake of keratinocytes.
Protokolle
| PRODUKTSPEZIFISCHE PROTOKOLLE | |
|---|---|
| WB protocol for RPE65 antibody 83861-1-RR | Protokoll herunterladen |
| STANDARD-PROTOKOLLE | |
|---|---|
| Klicken Sie hier, um unsere Standardprotokolle anzuzeigen |
Rezensionen
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Alex (Verified Customer) (12-16-2024) | I have tested this RPE65 primary recombinant antibody used in western blotting, dilution factor 1 in 5000 as recommended in the website, used on ARPE-19 cells. I have clear bands with minimum non-specific bands at higher molecular weight, however, the clear bands correspond to ~95 kDa and not ~65 kDa.
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