RPGR Polyklonaler Antikörper

RPGR Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 16891-1-PBS

Synonyme

RP3, X linked retinitis pigmentosa GTPase regulator, X-linked retinitis pigmentosa GTPase regulator, XLRP3

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  WB analysis using 16891-1-AP (same clone as 16891-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  WB analysis using 16891-1-AP (same clone as 16891-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 16891-1-AP (same clone as 16891-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 16891-1-AP (same clone as 16891-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  WB analysis of Y79 using 16891-1-AP (same clone as 16891-1-PBS)

  Y79 cells were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  WB analysis of SH-SY5Y using 16891-1-AP (same clone as 16891-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16891-1-AP (RPGR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 16891-1-AP (RPGR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse eye using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 16891-1-AP (RPGR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16891-1-AP (same clone as 16891-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16891-1-AP (RPGR Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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  IF Staining of hTERT-RPE1 cells using 16891-1-AP (same clone as 16891-1-PBS)

  IF result of anti-RPGR (16891-1-AP) in hTERT-RPE1 cell by Dr. Seongjin Seo. This data was developed using the same antibody clone with 16891-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

16891-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA RPGR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: RPGR fusion protein Ag10405
• Vollständiger Name: retinitis pigmentosa GTPase regulator
• Berechnetes Molekulargewicht: 113 kDa
• Beobachtetes Molekulargewicht: 100-105 kDa, 70 kDa
• GenBank-Zugangsnummer: BC031624
• Gene symbol: RPGR
• Gene ID (NCBI): 6103
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

RPGR is a protein with a series of six RCC1-like domains (RLDs), characteristic of the highly conserved guanine nucleotide exchange factors. The encoded protein is found in the Golgi body and interacts with RPGRIP1. This protein localizes to the outer segment of rod photoreceptors and is essential for their viability. Mutations in this gene have been associated with X-linked retinitis pigmentosa (XLRP). Multiple alternatively spliced transcript variants that encode different isoforms of this gene have been reported, but the full-length natures of only some have been determined. This antibody can recognize all the isoforms of RPGR.